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Definition

Designing a hydration study

The protocol should identify the tested formula, application amount and site, participant eligibility, acclimatisation conditions and any restrictions on other products. Baseline readings and an untreated site, vehicle or reference product help interpret changes. Temperature, humidity and measurement technique need control because they can influence instrument readings.

A duration claim requires measurements that actually cover the stated period and conditions of use. Repeated-use benefits and single-application effects are different questions. Sample size and analysis should be justified for the chosen endpoints, with variability and any missing observations addressed in the report.

Applications

Interpreting the deliverable

The study report should provide the protocol, measurement time points, numerical results, comparator analysis and a conclusion linked to the tested claim. Where percentages are presented, the calculation basis should be explicit. Participant questionnaires may add perceived comfort information but should remain distinguishable from instrumental results.

Transepidermal water loss, or TEWL, measures water vapour flux from skin and may provide complementary barrier-related information. It is not interchangeable with capacitance, and a TEWL change alone does not establish barrier repair. Hydration evidence does not establish wrinkle reduction, universal skin tolerance or market authorisation. Claim use must be reviewed for the destination market and remain within the study’s findings.

Exploration
Learn more about Hydration Barrier Testing

Explore human skin hydration and barrier testing, including water-loss assessments, alongside laboratory studies of epidermal lipids and water handling. Explore another Hydration Barrier Testing page on cosmetic-labs.com.

Barrier & Hydration Mechanisms

Investigate barrier and hydration mechanisms through epidermal lipids, junction proteins, differentiation and water-handling responses in skin models.

Skin Barrier Efficacy Testing

Measure human skin barrier responses, including transepidermal water loss where appropriate, with study conditions matched to the intended claim.

Skin Hydration Efficacy Testing

Measure cosmetic moisturising performance with skin hydration studies, defined comparison conditions and evidence matched to your proposed claims.

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What does “Verified” mean?

Verified means the claimant’s company affiliation was checked.

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