---
title: "Anti-inflammatory mediators increase testing"
description: "Test whether ingredients increase selected anti-inflammatory mediators."
image: "https://cosmetic-labs.com/wp-content/uploads/anti-inflammatory-service-1200x1200-1.webp"
---
# Anti-inflammatory mediators increase testing

> Test whether ingredients increase selected anti-inflammatory mediators.

Source: https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/anti-inflammatory
Created: 2025-02-11T11:04:23+00:00
Modified: 2026-10-25T18:46:52+00:00
Author: Emilie SCHOL

Anti-inflammatory mediators increase testing | Cosmetic Labs

## What is Anti-inflammatory mediators increase testing?

Anti-inflammatory mediator increase testing investigates whether an ingredient raises signals involved in limiting or resolving inflammation. Defined regulatory endpoints, such as IL-10 where appropriate to the model, distinguish this mechanism from suppression of pro-inflammatory cytokines.

### Why do you need this service?
Use regulatory-mediator studies to investigate whether treatment increases a specified signal involved in limiting or resolving inflammation. Distinguish that endpoint from simply reducing pro-inflammatory output.

## Be the first company on this service page

No published company profiles are linked to Anti-inflammatory mediators increase testing or its more specific services yet. Explore the service categories or add an explicitly supported capability to your company profile.

[List your company](https://cosmetic-labs.com/account/companies/new)
Definition
## Increasing anti-inflammatory mediators
Inflammatory regulation includes production of mediators that limit a response or support its resolution. This service asks whether treatment increases selected regulatory signals, rather than merely lowers markers such as TNF-α or IL-6.

### Model and endpoint selection
The cell system must be capable of producing the mediator of interest. For example, IL-10 may be relevant in an appropriate immune-cell or co-culture model. A mediator’s role depends on context, so no broad cytokine increase should automatically be labeled anti-inflammatory.

qPCR for expression of selected regulatory genes
- ELISA or multiplex assays for mediator proteins
- Cellular phenotyping where needed to identify the responding population
- Viability and appropriate baseline or challenge controls

### Functional follow-up
Concentration-response and time-course studies characterize the effect. Parallel measurements of inflammatory output can help test whether an increase accompanies reduced inflammatory activity, but those are complementary outcomes rather than substitutes for the stated endpoint.

Results can guide mechanism studies and selection of ingredients for soothing formulations. They do not independently prove barrier repair, clinical irritation reduction, or treatment of inflammatory skin disease. Reports should identify the mediator, model, controls, and limitations of the interpretation.

Applications
## Applications of anti-inflammatory mediator studies

### Measuring a regulatory response
Choose cells capable of producing the mediator of interest. In a suitable system, IL-10-related transcript and protein measurements can address different levels of response. IL-1β and TNF-α are complementary inflammatory readouts, not substitutes for demonstrating increased regulatory mediators.

### Testing functional context
Concentration-response comparisons and viability checks can be paired with inflammatory-output measurements. A mediator change needs interpretation in its biological context. Laboratory findings can guide soothing-formula research, but do not establish clinically proven calming, a quantified human inflammation reduction or treatment of inflammatory disease.

ExplorationLearn more about Inflammation & Skin ImmunityInvestigate skin inflammation and innate immune responses through cytokines, signalling pathways and cell assays suited to the research question. Explore another Inflammation & Skin Immunity page on cosmetic-labs.com.

#### [Sensitive skin and skin comfort efficacy testing](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/sensitive-skin)
Investigate sensitive-skin mechanisms through TRPV activity, substance P signalling and neurogenic response assays in suitable laboratory models. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/sensitive-skin)
#### [TRPV receptors modulation](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/trpv-receptors)
TRPV receptor assays measure channel activity and cellular responses to compare cosmetic actives and investigate possible soothing mechanisms. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/trpv-receptors)
#### [Substance P regulation](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/substance-p)
Investigate how cosmetic ingredients affect substance P release or signalling in relevant models, with findings limited to the tested skin mechanism. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/substance-p)
#### [Neurogenic reduction](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/neurogenic-inflammation)
Neurogenic reduction studies measure nerve-mediated inflammatory signals in relevant models to investigate mechanisms for soothing cosmetics. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/neurogenic-inflammation)
#### [Skin immunity efficacy testing](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/skin-immunity)
Skin immunity testing examines receptor signals, antimicrobial peptides and immune-cell responses to investigate cosmetic ingredient activity. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/skin-immunity)
#### [Innate response activation](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/innate-immunity)
Innate response assays examine receptor signaling, cytokines and stress markers to assess ingredient-driven activation in skin or immune cells. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/innate-immunity)
#### [TLR modulation](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/toll-receptor)
TLR modulation testing measures receptor-specific signaling and cytokine responses to investigate cosmetic ingredient effects on immune pathways. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/toll-receptor)
#### [Dendritic cell function](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/dendritic-cell)
Dendritic cell assays measure activation markers, cytokines and viability to investigate cosmetic ingredient effects on immune-cell function. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/dendritic-cell)
#### [AMP production](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/antimicrobial-peptides)
AMP production assays measure antimicrobial peptide expression and abundance in skin models to investigate ingredient effects on skin defense. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/antimicrobial-peptides)
#### [β-defensin expression](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/beta-defensin)
Beta-defensin expression testing measures transcripts and peptide levels in skin models to investigate ingredient effects on defense signaling. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/beta-defensin)
#### [Skin Inflammation Mechanism Studies](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammatory-disorders)
Investigate skin inflammatory mechanisms through cytokines, signalling pathways and cell responses in defined models for soothing-product research. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammatory-disorders)
#### [Cytokine profile modulation](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/cytokine-profile)
Cytokine profiling compares signaling mediators in cell or tissue models to investigate ingredient responses and guide sensitive-skin research. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/cytokine-profile)
#### [Cytokine cascade modulation](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammatory-cascade)
Measure ingredient effects on inflammatory cascades in skin-cell or tissue models exposed to a defined challenge, including UV where relevant. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammatory-cascade)
#### [NF-κB pathway inhibition](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/nfkb-inhibition)
Assess ingredient effects on NF-κB activation and downstream inflammatory gene expression in cell models relevant to soothing-formulation research. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/nfkb-inhibition)
#### [TNF-α/IL-6 suppression](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/cytokines-reduction)
Measure TNF-α and IL-6 responses in defined skin or immune-cell models, interpreting protein and transcript results alongside cell viability controls. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/cytokines-reduction)
#### [Eicosanoid mediator control](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammatory-mediators)
Investigate ingredient effects on prostaglandins and leukotrienes using enzyme or cell assays, with findings scoped to inflammatory signalling pathways. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammatory-mediators)
#### [COX-2 inhibition](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/cox2)
Measure COX-2 inhibition and related prostaglandin responses with enzyme or cell assays to investigate inflammatory mechanisms in cosmetic research. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/cox2)
#### [Prostaglandin synthesis regulation](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/prostaglandin)
Investigate prostaglandin synthesis and regulation in laboratory models to assess ingredient effects on inflammatory pathways relevant to skin comfort. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/prostaglandin)
#### [Mast cell stabilization](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/mast-cell)
Assess mast-cell activation and degranulation through histamine release and related markers in laboratory models relevant to reactive-skin research. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/mast-cell)
#### [Inflammaging](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammaging)
Investigate persistent, low-grade inflammatory responses associated with skin ageing through cytokine, stress and senescence markers in defined models. [Check this service](https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/inflammaging)

Continue with

[Google](https://cosmetic-labs.com/?lean_auth=start&provider=google&redirect_to=https://cosmetic-labs.com/&error_return=https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/anti-inflammatory)
[LinkedIn](https://cosmetic-labs.com/?lean_auth=start&provider=linkedin&redirect_to=https://cosmetic-labs.com/&error_return=https://cosmetic-labs.com/services/clinical/efficacy/skin-comfort-testing/inflammation-skin-immunity/anti-inflammatory)

What does “Verified” mean?
Verified means the claimant’s company affiliation was checked.

Something not right?Let us know if any information on this page is incorrect or if you have concerns about who manages it.

[Report an issue](https://cosmetic-labs.com/platform/contact)

© 2026 Cosmetic Labs. All rights reserved.

---

## Site identity

- Organization: Cosmetic Labs
- Description: Cosmetic Labs is a directory and a platform to meet all your cosmetic product development needs. From sourcing raw material to finding the best CRO for your needs.
- Website: https://cosmetic-labs.com

```json
[{"@type":"WebPage","name":"Anti-inflammatory mediators increase testing","url":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity\/anti-inflammatory","mainEntity":{"@id":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity\/anti-inflammatory#service"},"isPartOf":{"@id":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity#webpage"},"breadcrumb":{"@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/cosmetic-labs.com\/"},{"@type":"ListItem","position":2,"name":"Services","item":"https:\/\/cosmetic-labs.com\/services"},{"@type":"ListItem","position":3,"name":"Efficacy & Claims","item":"https:\/\/cosmetic-labs.com\/services\/clinical"},{"@type":"ListItem","position":4,"name":"Cosmetic Efficacy Testing","item":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy"},{"@type":"ListItem","position":5,"name":"Skin Comfort Testing","item":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing"},{"@type":"ListItem","position":6,"name":"Inflammation & Skin Immunity","item":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity"},{"@type":"ListItem","position":7,"name":"Anti-inflammatory mediators increase testing","item":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity\/anti-inflammatory"}]},"inLanguage":"en-US","@id":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity\/anti-inflammatory#webpage"},{"@type":"Service","name":"Anti-inflammatory mediators increase testing","url":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity\/anti-inflammatory","mainEntityOfPage":{"@id":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity\/anti-inflammatory#webpage"},"description":"Test whether ingredients increase selected anti-inflammatory mediators.","image":{"@type":"ImageObject","url":"https:\/\/cosmetic-labs.com\/wp-content\/uploads\/anti-inflammatory-service-1200x1200-1-1024x1024.webp","width":1024,"height":1024,"caption":"A teal-gloved hand positions capped tube strips above a slotted metal holder beside a separate sample rack."},"@id":"https:\/\/cosmetic-labs.com\/services\/clinical\/efficacy\/skin-comfort-testing\/inflammation-skin-immunity\/anti-inflammatory#service"}]
```